Title |
Identificación de micobacterias en medio sólido mediante microscopía de fase invertida y tinción Ziehl-Neelsen
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Published in |
Revista Peruana de Medicina Experimental y Salud Pública, June 2018
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DOI | 10.17843/rpmesp.2018.352.3471 |
Pubmed ID | |
Authors |
Jesús Rojas Jaimes, Jorge Giraldo-Chavez, Yudit Huyhua-Flores, Tatiana Caceres-Nakiche |
Abstract |
With the aim of identifying morphological differences in colonies of different species of mycobacteria by means of inverted phase microscopy, nine species of high-prevalence pathogenic mycobacteria were seeded in 7H11 solid medium and were observed for up to 21 days. Additionally, Ziehl-Neelsen (ZN) stains were carried out for each one of them. No morphological variations were identified among the species grown on solid medium; however, it was identified that all are able to form cords in solid medium in an early stage of growth, being corroborated by the Ziehl-Neelsen stain in Mycobacterium tuberculosis. so techniques based on detecting M. tuberculosis depending on the identification of the cords should be taken with caution so as not to generate a bad diagnosis, so microbiological characteristics could also be established as a minimum time to detect growth, temperature of growth and bacillary characteristics based on the Ziehl-Neelsen stain for each species studied. |
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