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Effects of drying and storage conditions on the stability of TSH in blood spots

Overview of attention for article published in Archives of Endocrinology and Metabolism, March 2018
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Title
Effects of drying and storage conditions on the stability of TSH in blood spots
Published in
Archives of Endocrinology and Metabolism, March 2018
DOI 10.20945/2359-3997000000026
Pubmed ID
Authors

Patrícia Künzle Ribeiro Magalhães, Carlos Henrique Miranda, Fernando Crivelenti Vilar, André Schmidt, Roberta Rodrigues Bittar, Giselle Aparecida Caixe de Carvalho Paixão, Edson Zangiacomi Martinez, Léa Maria Zanini Maciel

Abstract

To evaluate the influence of sample drying and storage temperature on TSH stability in neonatal screening. Blood samples from 29 adult volunteers as a surrogate for neonatal blood (10 with normal TSH, 9 with overt hypothyroid and 10 with subclinical hypothyroidism) were spotted on filter paper and dried at 22°C or 35°C for 3 hours. The samples were then stored at 22°C, -4°C, or -20°C, and TSH measurements were performed at day 0 (D0), D7, D30, D60, D180, and D360 of storage. The drying temperature did not interfere with TSH measurement on D0. TSH values remained stable up to D30 when stored at 22°C and were stable up to D60 when stored in a refrigerator or freezer. Samples stored at 22°C had a greater decrease in TSH values than samples stored in a refrigerator or a freezer. Freezer storage is not advantageous compared to storage in the refrigerator. At the end of one year, if confirmation of the initial result is required, a reduction of TSH concentrations should be taken into account.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 22 100%

Demographic breakdown

Readers by professional status Count As %
Student > Doctoral Student 4 18%
Other 2 9%
Student > Bachelor 2 9%
Unspecified 1 5%
Professor 1 5%
Other 5 23%
Unknown 7 32%
Readers by discipline Count As %
Medicine and Dentistry 5 23%
Nursing and Health Professions 2 9%
Psychology 2 9%
Immunology and Microbiology 1 5%
Unspecified 1 5%
Other 2 9%
Unknown 9 41%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 July 2018.
All research outputs
#20,527,576
of 23,096,849 outputs
Outputs from Archives of Endocrinology and Metabolism
#205
of 269 outputs
Outputs of similar age
#292,872
of 331,621 outputs
Outputs of similar age from Archives of Endocrinology and Metabolism
#10
of 11 outputs
Altmetric has tracked 23,096,849 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 269 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 11 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.