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He later developed the ACME dissociation method to preserve high-quality RNA, combining it with SPLiT-seq for high-resolution single-cell data (https://t.co/nkOWgb1meM). (2/3)
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This paper is key for me and I am indebted to them for publishing it. Rafa was my thesis advisor and Jaume was his. Our first lab paper explored the maceration solution for single cell transcriptomics (now ACME). We now explore cell type allometry. htt…
2,643 followers
@AArgoetti Reducing agents are very good at inhibiting RNAses, but if you need in your assay a functioning protein with even a single disulfide bridge, it'll be a problem. See for example NAC in this protocol: https://t.co/IQdBDZa6aR
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RT @KKo1wa1: @2shitsuJI @yoshidamasaaki 3'ならメタノール固定のものがあります🫡 https://t.co/aomZjRYHV3 これも良さそうです。 https://t.co/wWwi03k8YL