Title |
Immunostimulatory in Vitro and in Vivo Effects of a Water-Soluble Extract from Kale
|
---|---|
Published in |
Nippon Nōgeikagaku Kaishi, May 2014
|
DOI | 10.1271/bbb.100490 |
Pubmed ID | |
Authors |
Kosuke NISHI, Ai KONDO, Takeaki OKAMOTO, Hiroyuki NAKANO, Miho DAIFUKU, Sogo NISHIMOTO, OCHI Kenji, Terumi TAKAOKA, Takuya SUGAHARA |
Abstract |
The water-soluble fraction of kale (Brassica oleracea L. var. acephala DC.) had immunoglobulin (Ig) production stimulating activity in human hybridoma HB4C5 cells and human peripheral blood lymphocytes. The biochemical and physical properties of the main active substance in kale were found to be a heat-stable protein with a molecular weight higher than 50 kDa. The Ig production-stimulating factors were assumed to act on the translational and/or secreting processes of Igs. This Ig production-stimulating effect was also observed in lymphocytes from the mesenteric lymph node and Peyer's patches of mice that had been administered with the kale extract for 14 d. The partially purified kale extract was analyzed by LC-ESI-MS/MS, the result indicating ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) as an active substance. Rubisco from spinach indeed exhibited Ig production-stimulating activity in HB4C5 cells. These findings provide another beneficial aspect of kale as a health-promoting foodstuff. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 22 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 4 | 18% |
Researcher | 3 | 14% |
Student > Bachelor | 3 | 14% |
Student > Master | 2 | 9% |
Student > Doctoral Student | 1 | 5% |
Other | 5 | 23% |
Unknown | 4 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 10 | 45% |
Medicine and Dentistry | 2 | 9% |
Nursing and Health Professions | 2 | 9% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 5% |
Biochemistry, Genetics and Molecular Biology | 1 | 5% |
Other | 2 | 9% |
Unknown | 4 | 18% |