Accumulation of biogenic amines (BAs) in cheese and other foods is a matter of public health concern. This study aimed at identifying enzyme activities responsible for BAs degradation in lactic acid bacteria, which were previously isolated from traditional Sicilian and Apulian cheeses. Selected strains would control the concentration of BAs during cheese manufacture. First, 431 isolates, not showing genes encoding for decarboxylases responsible for BA formation, were selected using PCR-based methods. Ninety-four out of the 431 isolates degraded BAs (2-phenylethylamine, cadaverine, histamine, putrescine, spermine, spermidine, tyramine or tryptamine) during cultivation on chemically defined medium. As shown by RAPD-PCR and partial sequence of 16S-rRNA gene, 78 of the 94 strains belonged to Lactobacillus (Lb. casei, Lb. fermentum, Lb. parabuchneri, Lb. paracasei, Lb. paraplantarum and Lb. rhamnosus), Leuconostoc (Ln. lactis and Ln. mesenteroides), Pediococcus pentosaceus, Lactococcus lactis, Streptococcus (S. gallolyticus and S. thermophilus), Enterococcus lactis and Weissella paramesenteroidesA multi-copper oxidase hydrolyzing BAs was purified from the most active strain Lb. paracasei subsp. paracasei CB9CT. The gene encoding for the multi-copper oxidase was sequenced and detected also in other amine-degrading strains of Lb. fermentum, Lb. paraplantarum and P. pentosaceus Lb. paracasei CB9CT and another strain (CACIO6CT) of the same species that was able to degrade all the BAs were singly used as adjunct starter for decreasing the concentration of histamine and tyramine in industrial Caciocavallo cheese. The results of this study disclose a feasible strategy for increasing the safety of traditional cheeses, while maintaining their typical sensorial traits.
Because high concentrations of the potentially toxic biogenic amines may be found in traditional/typical cheeses, safety of these food items should be improved. Lactic acid bacteria selected for the ability to degrade biogenic amines may be used during cheese-making for controlling over the concentration of biogenic amines.
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 August 2022.
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#10,152,121
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Outputs from Applied and Environmental Microbiology
#9,298
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#117,596
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Outputs of similar age from Applied and Environmental Microbiology
#52
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Altmetric has tracked 30,385,879 research outputs across all sources so far. This one has received more attention than most of these and is in the 65th percentile.
So far Altmetric has tracked 19,879 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.4. This one has gotten more attention than average, scoring higher than 52% of its peers.
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