Study Design Controlled Laboratory Study; Cross Sectional. Background Little is known about ankle range of motion (ROM) and strength among patients with insertional Achilles tendinopathy (IAT) and whether limited ankle ROM and plantarflexor weakness impact IAT symptom severity. Objectives The purposes of the study were to 1) examine if participants with IAT exhibit limited non-weight-bearing dorsiflexion ROM, reduced plantarflexor strength and/or altered ankle biomechanics during stair ascent, and 2) determine which impairments are associated with symptom severity. Methods Participants included 20 patients with unilateral IAT (Age=59 ± 8 years, 55% female) and 20 individuals without tendinopathy (Age=58.2 ± 8.5 years, 55% female). A dynamometer was used to measure non-weight-bearing ROM and isometric plantarflexor strength. 3D motion analysis was used to quantify ankle biomechanics during stair ascent. End-range dorsiflexion was quantified as the percentage of non-weight-bearing dorsiflexion used during stair ascent. Group differences were compared using 2-way and 1-way ANOVAs. Pearson correlations were used to test for associations among dependent variables and symptom severity. Results Groups differed in ankle biomechanics but not non-weight-bearing ROM or strength. During stair ascent the IAT group used greater end-range dorsiflexion (P=0.03), less plantar flexion (P=0.02), and lower peak ankle plantarflexor power (P=0.01) than the control group. Higher end-range dorsiflexion and lower ankle power during stair ascent were associated with greater symptom severity (P<0.05). Conclusions Patients with IAT do not experience restrictions in non-weight-bearing dorsiflexion ROM or isometric plantarflexor strength. However, altered ankle biomechanics during stair ascent were linked with greater symptom severity and likely contribute to decreased function. J Orthop Sports Phys Ther, Epub 29 Oct 2016. doi:10.2519/jospt.2016.6462.