| Title |
Modular Scanning Confocal Microscope with Digital Image Processing
|
|---|---|
| Published in |
PLOS ONE, November 2016
|
| DOI | 10.1371/journal.pone.0166212 |
| Pubmed ID | |
| Authors | |
| Abstract |
In conventional confocal microscopy, a physical pinhole is placed at the image plane prior to the detector to limit the observation volume. In this work, we present a modular design of a scanning confocal microscope which uses a CCD camera to replace the physical pinhole for materials science applications. Experimental scans were performed on a microscope resolution target, a semiconductor chip carrier, and a piece of etched silicon wafer. The data collected by the CCD were processed to yield images of the specimen. By selecting effective pixels in the recorded CCD images, a virtual pinhole is created. By analyzing the image moments of the imaging data, a lateral resolution enhancement is achieved by using a 20 × / NA = 0.4 microscope objective at 532 nm laser wavelength. |
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|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
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Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 19 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 26% |
| Researcher | 4 | 21% |
| Professor | 2 | 11% |
| Student > Doctoral Student | 1 | 5% |
| Student > Bachelor | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 5 | 26% |
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|---|---|---|
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| Engineering | 3 | 16% |
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| Chemistry | 2 | 11% |
| Psychology | 1 | 5% |
| Other | 0 | 0% |
| Unknown | 6 | 32% |