@jmdubs6 @ImmunoFever @plasmidsaurus Low quality, drop out ridden RNAseq is just contaminating science with false "specific" gene expression Some reason why, here: https://t.co/0X61hYF6Qo
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2,112 followers
@MariosGeorgakis Note that most RNAseq data is devoid of reliable canonical ncRNA measures due to the failures of the cDNA library step https://t.co/FqA8tmdTQo intergenic DNA regulatory elements even trickier.
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@fumikawano We have repeatedly shown (in metabolic tissues) claims of specific (eg pancreas) lncRNA are false We've shown that RNAseq produce a small at random sub sample of what the tissue actually expresses - muscle example here https://t.co/FqA8tmdTQ…
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@blekhman Did granny mention early Illumina short read seq scammed its way to "bigger unbiased coverage"? Oh yeh and the QC consortium in 2016 reported transcript counts as "genes". https://t.co/0X61hYF6Qo