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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Improvement of recovery yield of macro-organismal environmental DNA from seawater samples
|
|---|---|
| Published in |
Analytical Sciences, February 2023
|
| DOI | 10.1007/s44211-023-00280-1 |
| Pubmed ID | |
| Authors |
Qianqian Wu, Toshifumi Minamoto |
| Abstract |
In recent years, environmental DNA (eDNA) technology has been used in a variety of water environments. Environmental DNA concentrations in marine samples tend to be lower than those in freshwater samples, and few studies have explored methods to improve the recovery yields of eDNA from seawater samples. In this study, we compared different seawater preservation solutions (RNAlater or ATL) to improve eDNA yields. The eDNA concentrations of vertebrate and invertebrate species were compared using species-specific eDNA assays, and the number of detected fish and their compositions were compared using metabarcoding analysis. ATL treatment resulted in significantly higher eDNA yields for both vertebrate and invertebrate species than RNAlater treatment. Metabarcoding analysis revealed non-significant effects of preservation on the number of detected species and species composition. These results suggest that ATL treatment improves DNA yields without changing the species composition compared with the commonly used RNAlater treatment. The findings of this study will reduce false-negative outcomes and provide highly reliable results in future biological surveys. |
X Demographics
The data shown below were collected from the profiles of 15 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 2 | 13% |
| Mexico | 1 | 7% |
| United Kingdom | 1 | 7% |
| Brazil | 1 | 7% |
| Belgium | 1 | 7% |
| Unknown | 9 | 60% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 14 | 93% |
| Scientists | 1 | 7% |
Mendeley readers
The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 24 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 21% |
| Student > Doctoral Student | 4 | 17% |
| Other | 2 | 8% |
| Student > Ph. D. Student | 2 | 8% |
| Student > Master | 2 | 8% |
| Other | 1 | 4% |
| Unknown | 8 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 9 | 38% |
| Biochemistry, Genetics and Molecular Biology | 4 | 17% |
| Immunology and Microbiology | 1 | 4% |
| Economics, Econometrics and Finance | 1 | 4% |
| Psychology | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 8 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 January 2024.
All research outputs
#4,347,045
of 25,905,864 outputs
Outputs from Analytical Sciences
#42
of 1,176 outputs
Outputs of similar age
#84,567
of 480,533 outputs
Outputs of similar age from Analytical Sciences
#1
of 53 outputs
Altmetric has tracked 25,905,864 research outputs across all sources so far. Compared to these this one has done well and is in the 83rd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,176 research outputs from this source. They receive a mean Attention Score of 2.8. This one has done particularly well, scoring higher than 96% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 480,533 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 82% of its contemporaries.
We're also able to compare this research output to 53 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 98% of its contemporaries.