Title |
Biochemical Analysis of Plant Protection Afforded by a Nonpathogenic Endophytic Mutant of Colletotrichum magna
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Published in |
Plant Physiology, February 1999
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DOI | 10.1104/pp.119.2.795 |
Pubmed ID | |
Authors |
Regina S. Redman, Stanley Freeman, David R. Clifton, Jed Morrel, Gayle Brown, Rusty J. Rodriguez |
Abstract |
A nonpathogenic mutant of Colletotrichum magna (path-1) was previously shown to protect watermelon (Citrullus lanatus) and cucumber (Cucumis sativus) seedlings from anthracnose disease elicited by wild-type C. magna. Disease protection was observed in stems of path-1-colonized cucurbits but not in cotyledons, indicating that path-1 conferred tissue-specific and/or localized protection. Plant biochemical indicators of a localized and systemic (peroxidase, phenylalanine ammonia-lyase, lignin, and salicylic acid) "plant-defense" response were investigated in anthracnose-resistant and -susceptible cultivars of cucurbit seedlings exposed to four treatments: (1) water (control), (2) path-1 conidia, (3) wild-type conidia, and (4) challenge conditions (inoculation into path-1 conidia for 48 h and then exposure to wild-type conidia). Collectively, these analyses indicated that disease protection in path-1-colonized plants was correlated with the ability of these plants to mount a defense response more rapidly and to equal or greater levels than plants exposed to wild-type C. magna alone. Watermelon plants colonized with path-1 were also protected against disease caused by Colletotrichum orbiculare and Fusarium oxysporum. A model based on the kinetics of plant-defense activation is presented to explain the mechanism of path-1-conferred disease protection. |
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Researcher | 16 | 15% |
Student > Bachelor | 9 | 9% |
Professor | 7 | 7% |
Other | 21 | 20% |
Unknown | 13 | 13% |
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Immunology and Microbiology | 2 | 2% |
Other | 4 | 4% |
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