Title |
Liquid chromatographic determination of benzocaine and N-acetylbenzocaine in the edible fillet tissue from rainbow trout
|
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Published in |
Journal of Chromatography A, September 1999
|
DOI | 10.1016/s0021-9673(99)00665-2 |
Pubmed ID | |
Authors |
Jeffery R Meinertz, Guy R Stehly, Terrance D Hubert, Jeffry A Bernardy |
Abstract |
A method was developed for determining benzocaine and N-acetylbenzocaine concentrations in fillet tissue of rainbow trout. The method involves extracting the analytes with acetonitrile, removing lipids or hydrophobic compounds from the extract with hexane, and providing additional clean-up with solid-phase extraction techniques. Analyte concentrations are determined using reversed-phase high-performance liquid chromatographic techniques with an isocratic mobile phase and UV detection. The accuracy (range, 92 to 121%), precision (R.S.D., < 14%), and sensitivity (method quantitation limit, < 24 ng/g) for each analyte indicate the usefulness of this method for studies characterizing the depletion of benzocaine residues from fish exposed to benzocaine. |
Mendeley readers
Geographical breakdown
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United States | 1 | 6% |
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Demographic breakdown
Readers by professional status | Count | As % |
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Unknown | 2 | 13% |
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Other | 0 | 0% |
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