Title |
Immortalized Bovine Satellite Cells for Cultured Meat Applications
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Published in |
ACS Synthetic Biology, May 2023
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DOI | 10.1021/acssynbio.3c00216 |
Pubmed ID | |
Authors |
Andrew J. Stout, Miles J. Arnett, Kristin Chai, Tina Guo, Lishu Liao, Addison B. Mirliani, Miriam L. Rittenberg, Michelle Shub, Eugene C. White, John S. K. Yuen, Xiaoli Zhang, David L. Kaplan |
Abstract |
For cultured meat to succeed at scale, muscle cells from food-relevant species must be expanded in vitro in a rapid and reliable manner to produce millions of metric tons of biomass annually. Toward this goal, genetically immortalized cells offer substantial benefits over primary cells, including rapid growth, escape from cellular senescence, and consistent starting cell populations for production. Here, we develop genetically immortalized bovine satellite cells (iBSCs) via constitutive expression of bovine Telomerase reverse transcriptase (TERT) and Cyclin-dependent kinase 4 (CDK4). These cells achieve over 120 doublings at the time of publication and maintain their capacity for myogenic differentiation. They therefore offer a valuable tool to the field, enabling further research and development to advance cultured meat. |
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Geographical breakdown
Country | Count | As % |
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United States | 7 | 17% |
Japan | 2 | 5% |
Poland | 2 | 5% |
India | 1 | 2% |
Sweden | 1 | 2% |
Israel | 1 | 2% |
Canada | 1 | 2% |
United Kingdom | 1 | 2% |
France | 1 | 2% |
Other | 1 | 2% |
Unknown | 23 | 56% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 32 | 78% |
Scientists | 7 | 17% |
Practitioners (doctors, other healthcare professionals) | 2 | 5% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 65 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 12 | 18% |
Student > Bachelor | 8 | 12% |
Student > Ph. D. Student | 7 | 11% |
Student > Doctoral Student | 3 | 5% |
Student > Master | 3 | 5% |
Other | 3 | 5% |
Unknown | 29 | 45% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 16 | 25% |
Agricultural and Biological Sciences | 9 | 14% |
Medicine and Dentistry | 2 | 3% |
Engineering | 2 | 3% |
Unspecified | 1 | 2% |
Other | 4 | 6% |
Unknown | 31 | 48% |