Myogenesis is positively regulated by thyroid hormone (T3), which is amplified by the type 2 deiodinase (D2) activation of T4-to-T3. Global inactivation of Dio2 gene impairs skel-etal muscle (SKM) differentiation and regeneration in response to muscle injury. Given that new-born and adult mice with late developmental SKM Dio2 disruption do not develop a significant phenotype, we hypothesized that D2 plays an early role in this process.
This was tested in mice with SKM disruption of Dio2 driven by two early develop-mental promoters, i.e. MYF5 or MYOD.
MYF5 myoblasts in culture differentiate normally into myotubes despite loss of almost all D2 activity. Dio2 mRNA levels in developing skeletal muscle obtained from MYF5-D2KO embryos (E18.5) were about 54% of control littermates but the expression of T3-responsive genes Myh1 and 7, Atp2a1 and 2 were not affected. In MYF5-D2KO and MYOD-D2KO neo-natal hind limb muscle the expression of Myh1 and 7, Atp2a2 remained unaffected despite 60-70% loss in D2 activity and/or mRNA. Only in MYOD-D2KO neonatal muscle there was a 40% reduction in Atp2a1 mRNA. Post-natal growth of both mouse models and skeletal muscle func-tion as assessed by exercise capacity and measurement of muscle strength were normal. Further-more, an analysis of the adult soleus (SOL) revealed no changes in the expression of T3-responsive genes, except for an about 18% increase in MYOD-D2KO SOL Myh7 mRNA.
Two mouse models of early developmental disruption of Dio2 in myocyte precursor exhibit no significant skeletal muscle phenotype.
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 February 2018.
All research outputs
#16,048,318
of 25,377,790 outputs
Outputs from Thyroid
#1,440
of 2,211 outputs
Outputs of similar age
#243,853
of 427,386 outputs
Outputs of similar age from Thyroid
#22
of 29 outputs
Altmetric has tracked 25,377,790 research outputs across all sources so far. This one is in the 34th percentile – i.e., 34% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,211 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.7. This one is in the 32nd percentile – i.e., 32% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 427,386 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 40th percentile – i.e., 40% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 29 others from the same source and published within six weeks on either side of this one. This one is in the 24th percentile – i.e., 24% of its contemporaries scored the same or lower than it.
