Title |
Cell-type specific defects in PTEN-mutant cortical organoids converge on abnormal circuit activity
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Published in |
Human Molecular Genetics, June 2023
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DOI | 10.1093/hmg/ddad107 |
Pubmed ID | |
Authors |
Martina Pigoni, Ana Uzquiano, Bruna Paulsen, Amanda J Kedaigle, Sung Min Yang, Panagiotis Symvoulidis, Xian Adiconis, Silvia Velasco, Rafaela Sartore, Kwanho Kim, Ashley Tucewicz, Sarah Yoshimi Tropp, Kalliopi Tsafou, Xin Jin, Lindy Barrett, Fei Chen, Edward S Boyden, Aviv Regev, Joshua Z Levin, Paola Arlotta |
Abstract |
De novo heterozygous loss-of-function mutations in PTEN are strongly associated with Autism spectrum disorders (ASD); however, it is unclear how heterozygous mutations in this gene affects different cell types during human brain development, and how these effects vary across individuals. Here, we used human cortical organoids from different donors to identify cell-type-specific developmental events that are affected by heterozygous mutations in PTEN. We profiled individual organoids by single-cell RNA-seq, proteomics, and spatial transcriptomics, and revealed abnormalities in developmental timing in human outer radial glia progenitors and deep layer cortical projection neurons, which varied with the donor genetic background. Calcium imaging in intact organoids showed that both accelerated and delayed neuronal development phenotypes resulted in similar abnormal activity of local circuits, irrespective of genetic background. The work reveals donor-dependent, cell-type specific developmental phenotypes of PTEN heterozygosity that later converge on disrupted neuronal activity. |
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Professor | 2 | 13% |
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Student > Bachelor | 1 | 7% |
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Unspecified | 1 | 7% |
Other | 0 | 0% |
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