The non-classical progesterone receptors, progesterone receptor membrane component (PGRMC) 1 and PGRMC2 have been implicated in regulating cell survival of endometrial and ovarian cells in vitro and are abundantly expressed in these cell types. The objective of this study was to determine if Pgrmc1 and Pgrmc2 are essential for normal female reproduction. To accomplish this objective, Pgrmc1 and/or Pgrmc2 floxed mice (Pgrmc2(fl/fl) and Pgrmc1/2(fl/fl)) were crossed with Pgr-cre mice which resulted in the conditional ablation of Pgrmc1 and/or Pgrmc2 from female reproductive tissues (i.e., Pgrmc2(d/d) and Pgrmc1/2(d/d) mice). A breeding trial revealed that conditional ablation of Pgrmc2 initially led to subfertility with Pgrmc2(d/d) female mice producing 47% fewer pups/litter than Pgrmc2(fl/fl) mice (p=0.001). Pgrmc2(d/d) mice subsequently underwent premature reproductive senescence by parities 2-5, producing 37.8% fewer litters overall during the trial compared to Pgrmc2(fl/fl) mice (p=0.020). Similar results were observed with Pgrmc1/2(d/d) mice. Based on ovarian morphology and serum P4 the subfertility/infertility was not due to faulty ovulation or luteal insufficiency. Rather an analysis of mid-gestation implantation sites revealed that post-implantation embryonic death was the major cause of the subfertility/infertility. As with our previous report of Pgrmc1(d/d) mice, Pgrmc2(d/d) and Pgrmc1/2(d/d) mice developed endometrial cysts consistent with accelerated aging of this tissue. Given the timing of post-implantation embryonic demise, uterine decidualization may be disrupted in mice deficient in PGRMC2 or PGRMC1/2. Overall, this study revealed that Pgrmc1 and/or Pgrmc2 are required for the maintenance of uterine histoarchitecture and normal female reproductive lifespan.
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 December 2016.
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