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Rapid Polymerase Chain Reaction Diagnosis of White-Nose Syndrome in Bats

Overview of attention for article published in Journal of Veterinary Diagnostic Investigation, March 2010
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Among the highest-scoring outputs from this source (#29 of 1,571)
  • High Attention Score compared to outputs of the same age (90th percentile)
  • High Attention Score compared to outputs of the same age and source (87th percentile)

Mentioned by

news
1 news outlet
blogs
1 blog

Citations

dimensions_citation
51 Dimensions

Readers on

mendeley
106 Mendeley
citeulike
1 CiteULike
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Title
Rapid Polymerase Chain Reaction Diagnosis of White-Nose Syndrome in Bats
Published in
Journal of Veterinary Diagnostic Investigation, March 2010
DOI 10.1177/104063871002200208
Pubmed ID
Authors

Jeffrey M. Lorch, Andrea Gargas, Carol Uphoff Meteyer, Brenda M. Berlowski-Zier, D. Earl Green, Valerie Shearn-Bochsler, Nancy J. Thomas, David S. Blehert

Abstract

A newly developed polymerase chain reaction (PCR)-based method to rapidly and specifically detect Geomyces destructans on the wings of infected bats from small quantities (1-2 mg) of tissue is described in the current study (methods for culturing and isolating G. destructans from bat skin are also described). The lower limits of detection for PCR were 5 fg of purified fungal DNA or 100 conidia per 2 mg of wing tissue. By using histology as the standard, the PCR had a diagnostic specificity of 100% and a diagnostic sensitivity of 96%, whereas the diagnostic sensitivity of culture techniques was only 54%. The accuracy and fast turnaround time of PCR provides field biologists with valuable information on infection status more rapidly than traditional methods, and the small amount of tissue required for the test would allow diagnosis of white-nose syndrome in live animals.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 106 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 8 8%
Hungary 1 <1%
Canada 1 <1%
Switzerland 1 <1%
Unknown 95 90%

Demographic breakdown

Readers by professional status Count As %
Researcher 27 25%
Student > Master 19 18%
Student > Ph. D. Student 16 15%
Student > Bachelor 12 11%
Other 9 8%
Other 16 15%
Unknown 7 7%
Readers by discipline Count As %
Agricultural and Biological Sciences 61 58%
Environmental Science 9 8%
Biochemistry, Genetics and Molecular Biology 6 6%
Veterinary Science and Veterinary Medicine 6 6%
Medicine and Dentistry 5 5%
Other 7 7%
Unknown 12 11%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 13. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 August 2011.
All research outputs
#2,390,586
of 22,711,242 outputs
Outputs from Journal of Veterinary Diagnostic Investigation
#29
of 1,571 outputs
Outputs of similar age
#9,314
of 94,028 outputs
Outputs of similar age from Journal of Veterinary Diagnostic Investigation
#1
of 8 outputs
Altmetric has tracked 22,711,242 research outputs across all sources so far. Compared to these this one has done well and is in the 89th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,571 research outputs from this source. They receive a mean Attention Score of 2.8. This one has done particularly well, scoring higher than 98% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 94,028 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 90% of its contemporaries.
We're also able to compare this research output to 8 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them