Title |
Rapid Polymerase Chain Reaction Diagnosis of White-Nose Syndrome in Bats
|
---|---|
Published in |
Journal of Veterinary Diagnostic Investigation, March 2010
|
DOI | 10.1177/104063871002200208 |
Pubmed ID | |
Authors |
Jeffrey M. Lorch, Andrea Gargas, Carol Uphoff Meteyer, Brenda M. Berlowski-Zier, D. Earl Green, Valerie Shearn-Bochsler, Nancy J. Thomas, David S. Blehert |
Abstract |
A newly developed polymerase chain reaction (PCR)-based method to rapidly and specifically detect Geomyces destructans on the wings of infected bats from small quantities (1-2 mg) of tissue is described in the current study (methods for culturing and isolating G. destructans from bat skin are also described). The lower limits of detection for PCR were 5 fg of purified fungal DNA or 100 conidia per 2 mg of wing tissue. By using histology as the standard, the PCR had a diagnostic specificity of 100% and a diagnostic sensitivity of 96%, whereas the diagnostic sensitivity of culture techniques was only 54%. The accuracy and fast turnaround time of PCR provides field biologists with valuable information on infection status more rapidly than traditional methods, and the small amount of tissue required for the test would allow diagnosis of white-nose syndrome in live animals. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 8 | 8% |
Hungary | 1 | <1% |
Canada | 1 | <1% |
Switzerland | 1 | <1% |
Unknown | 95 | 90% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 27 | 25% |
Student > Master | 19 | 18% |
Student > Ph. D. Student | 16 | 15% |
Student > Bachelor | 12 | 11% |
Other | 9 | 8% |
Other | 16 | 15% |
Unknown | 7 | 7% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 61 | 58% |
Environmental Science | 9 | 8% |
Biochemistry, Genetics and Molecular Biology | 6 | 6% |
Veterinary Science and Veterinary Medicine | 6 | 6% |
Medicine and Dentistry | 5 | 5% |
Other | 7 | 7% |
Unknown | 12 | 11% |