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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Comparison of a Multiplex Real-Time PCR Technique with Oxford Nanopore Technologies Next-Generation Sequencing for Identification of SARS-CoV-2 Variants of Concern
|
|---|---|
| Published in |
Intervirology, October 2023
|
| DOI | 10.1159/000534067 |
| Pubmed ID | |
| Authors |
Zahra Ahmadi, Ali Maleki, Sana Eybpoosh, Zahra Fereydouni, Mahsa Tavakoli, Setareh Kashanian, Laya Farhan Asadi, Amir Hesam Nemati, Mostafa Salehi-Vaziri |
| Abstract |
The rapid emergence of SARS-CoV-2 variants and their potential to endangering the global health, has increased the demand for a fast-tracking method in comparison to the Next-Generation-Sequencing (NGS) as a gold standard assay, particularly in developing countries. This study was designed to evaluate the performance of a commercial multiplex Real-Time PCR technique (GA SARS-CoV-2 OneStep RT-PCR Kit, Iran) for identification of SARS-CoV-2 variants of concern compared to Oxford Nanopore Next-Generation-Sequencing assay. A total of 238 SARS-CoV-2 positive respiratory samples from different waves of COVID-19 in Iran were randomly selected in this study. To determine the SARS-CoV-2 VOC, the samples were analyzed via the commercial triple target assay, GA SARS-CoV-2 OneStep RT-PCR Kit, and NGS as well. The results revealed good concordance between GA SARS-CoV-2 OneStep RT-PCR Kit and NGS for identification of SARS-CoV-2 VOCs. GA SARS-CoV-2 OneStep RT-PCR Kit identified Wuhan, Alpha and Delta variants with 100% relative sensitivity and specificity. Regarding Omicron subvariants of BA.1, BA.2 and BA.4/5 the relative sensitivity of 100%, 100% and 81.5% and the relative specificity of 95.3%, 93.5%, and 100% were observed. Overall, GA SARS-CoV-2 OneStep RT-PCR Kit can be used as a rapid and cost-effective alternative to NGS for identification of SARS-CoV-2 VOCs. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Unspecified | 1 | 17% |
| Student > Postgraduate | 1 | 17% |
| Student > Master | 1 | 17% |
| Unknown | 3 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Unspecified | 1 | 17% |
| Biochemistry, Genetics and Molecular Biology | 1 | 17% |
| Agricultural and Biological Sciences | 1 | 17% |
| Immunology and Microbiology | 1 | 17% |
| Unknown | 2 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 October 2023.
All research outputs
#20,021,843
of 24,605,383 outputs
Outputs from Intervirology
#585
of 675 outputs
Outputs of similar age
#123,251
of 179,643 outputs
Outputs of similar age from Intervirology
#1
of 3 outputs
Altmetric has tracked 24,605,383 research outputs across all sources so far. This one is in the 10th percentile – i.e., 10% of other outputs scored the same or lower than it.
So far Altmetric has tracked 675 research outputs from this source. They receive a mean Attention Score of 4.6. This one is in the 5th percentile – i.e., 5% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 179,643 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 19th percentile – i.e., 19% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 3 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them