In this study, binding of linear poly(L-lysine) to a series of acrylamide and 2-acrylamido-2-methyl-1-propanesulfonate copolymers was examined by isothermal titration calorimetry (ITC). Binding constant and stoichiometry were systematically determined at different ionic strengths and for different polyanion charge densities varying between 15% and 100%. The range of investigated ionic strengths was carefully adjusted according to the polyanion charge density to get measurable binding constants (i.e. formation binding constant typically comprised between 104 and 106 M-1) by isothermal titration calorimetry (ITC). The number of released counter-ions during the polyelectrolyte complex formation was determined from the log-log dependence of the binding constant according to the ionic strength, and was compared to the total number of condensed counter-ions estimated from the Manning theory. Experimental results obtained by ITC are in very good agreement with those previously obtained by frontal analysis continuous capillary electrophoresis (FACCE) and can be used to model and predict the binding parameters at any ionic strength or any polyanion charge density. Thermodynamic parameters of the complexation between the oppositely charged polyelectrolytes confirm that the complex formation was entropically driven together with a favourable (but minor) enthalpic contribution. For the first time, specificities, advantages / disadvantages of ITC and FACCE techniques for studying polyelectrolyte complexations are compared and discussed, using the same experimental conditions.
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 April 2021.
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#11,459,875
of 34,372,222 outputs
Outputs from Journal of Physical Chemistry B
#4,528
of 17,742 outputs
Outputs of similar age
#133,713
of 353,040 outputs
Outputs of similar age from Journal of Physical Chemistry B
#37
of 254 outputs
Altmetric has tracked 34,372,222 research outputs across all sources so far. This one has received more attention than most of these and is in the 65th percentile.
So far Altmetric has tracked 17,742 research outputs from this source. They receive a mean Attention Score of 3.5. This one has gotten more attention than average, scoring higher than 73% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 353,040 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 60% of its contemporaries.
We're also able to compare this research output to 254 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 80% of its contemporaries.
