Title |
In Vitro and In Vivo Uptake Study of Escherichia coli Nissle 1917 Bacterial Ghosts: Cell-Based Delivery System to Target Ocular Surface Diseases Escherichia coli Nissle 1917 Bacterial Ghosts
|
---|---|
Published in |
Investigative Ophthalmology & Visual Science, September 2013
|
DOI | 10.1167/iovs.13-12044 |
Pubmed ID | |
Authors |
Elisabeth Stein, Aleksandra Inic-Kanada, Sandra Belij, Jacqueline Montanaro, Nora Bintner, Simone Schlacher, Ulrike Beate Mayr, Werner Lubitz, Marijana Stojanovic, Hristo Najdenski, Talin Barisani-Asenbauer |
Abstract |
For the successful topical administration of drugs or vaccines to treat ocular surface diseases, efficient and well-tolerated delivery systems/carriers for conjunctival delivery are crucial in the development of new treatment strategies. The present study investigated the efficiency of internalization of bacterial ghosts (BGs) produced from probiotic Escherichia coli Nissle 1917 (EcN) by human conjunctival epithelial (HCjE) cell line, the EcN BGs cytotoxicity for HCjE cells, and in vivo uptake of EcN BGs by conjunctival guinea pig epithelial cells. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 39 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 10 | 26% |
Researcher | 6 | 15% |
Student > Ph. D. Student | 5 | 13% |
Professor > Associate Professor | 4 | 10% |
Student > Postgraduate | 2 | 5% |
Other | 3 | 8% |
Unknown | 9 | 23% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 8 | 21% |
Immunology and Microbiology | 7 | 18% |
Agricultural and Biological Sciences | 6 | 15% |
Biochemistry, Genetics and Molecular Biology | 5 | 13% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
Other | 2 | 5% |
Unknown | 10 | 26% |