@MingLi_Umich This is really cool, Ming - fantastic work! We did a screen for Pib1 substrates (Figure 7; https://t.co/glTwzLhWa0) and I concentrated on PM hits (Zeo1, Mup1, Gap1, Opt1) but there were some vacuolar hits too (Cps1, several V-ATPAse subunits…
Our approach to identify substrates / functions of E3-ubiquitin ligases is available in the July issue of Traffic: https://t.co/hIv5iVUoMw
Enzyme reversal to explore the function of yeast E3 ubiquitin-ligases https://t.co/wVkKcrGDX4
Pubmed: Enzyme reversal to explore the function of yeast E3 ubiquitin-ligases. https://t.co/wXeYxLyzHX