We investigated the antioxidative properties of (2R,3S,2''R,3''R)-Manniflavanone (MF) using in vitro assays, and examined its effects on myogenesis and lactate-induced oxidative stress in C2C12 cells. MF was purified from Garcinia buchananii stem bark. H2O2 and ORAC assay demonstrated that MF is a powerful antioxidant. This finding was supported by DPPH radical scavenging activity of MF. MF was less cytotoxic to C2C12 cells compared to ascorbic acid and myricetin. Moreover, MF accelerated myotube formation in the differentiated C2C12 cells by up-regulating myogenic proteins such as MyoG and MHC. Furthermore, MF rescued late differentiation of myoblast suppressed by lactate treatment and upregulated the expression levels of Nrf2 in lactate-induced oxidative stress, indicating that MF stimulates antioxidative activity inside C2C12 cells. Collectively, MF is a potent antioxidant with higher safety profile than ascorbic acid and myricetin. It reduces oxidative stress-induced delaying of skeletal muscle differentiation by scavenging ROS and regulating myogenic proteins factors.
Pharmacology, Toxicology and Pharmaceutical Science
1
9%
Physics and Astronomy
1
9%
Sports and Recreations
1
9%
Other
1
9%
Unknown
3
27%
Attention Score in Context
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 October 2019.
All research outputs
#11,903,285
of 34,372,222 outputs
Outputs from Journal of Agricultural & Food Chemistry
#10,718
of 25,822 outputs
Outputs of similar age
#138,833
of 356,187 outputs
Outputs of similar age from Journal of Agricultural & Food Chemistry
#76
of 198 outputs
Altmetric has tracked 34,372,222 research outputs across all sources so far. This one has received more attention than most of these and is in the 64th percentile.
So far Altmetric has tracked 25,822 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.3. This one has gotten more attention than average, scoring higher than 56% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 356,187 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 59% of its contemporaries.
We're also able to compare this research output to 198 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 59% of its contemporaries.
