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Optimization of PCR Conditions for Amplification of GC‐Rich EGFR Promoter Sequence

Overview of attention for article published in Journal of Clinical Laboratory Analysis, November 2013
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Title
Optimization of PCR Conditions for Amplification of GC‐Rich EGFR Promoter Sequence
Published in
Journal of Clinical Laboratory Analysis, November 2013
DOI 10.1002/jcla.21632
Pubmed ID
Authors

Jasmina Obradovic, Vladimir Jurisic, Natasa Tosic, Jasminka Mrdjanovic, Branislav Perin, Sonja Pavlovic, Natasa Djordjevic

Abstract

Polymerase chain reaction (PCR) is an extremely sensitive method that often demands optimization, especially when difficult templates need to be amplified. The aim of the present study was to optimize the PCR conditions for amplification of the epidermal growth factor receptor (EGFR) promoter sequence featuring an extremely high guanine-cytosine (GC) content in order to detect single nucleotide polymorphisms -216G>T and -191C>A.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 234 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 234 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 54 23%
Student > Master 18 8%
Student > Postgraduate 9 4%
Researcher 9 4%
Student > Ph. D. Student 8 3%
Other 17 7%
Unknown 119 51%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 56 24%
Agricultural and Biological Sciences 19 8%
Medicine and Dentistry 7 3%
Chemistry 5 2%
Pharmacology, Toxicology and Pharmaceutical Science 5 2%
Other 19 8%
Unknown 123 53%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 November 2013.
All research outputs
#20,655,488
of 25,371,288 outputs
Outputs from Journal of Clinical Laboratory Analysis
#881
of 1,453 outputs
Outputs of similar age
#169,603
of 225,254 outputs
Outputs of similar age from Journal of Clinical Laboratory Analysis
#2
of 3 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. This one is in the 10th percentile – i.e., 10% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,453 research outputs from this source. They receive a mean Attention Score of 3.2. This one is in the 27th percentile – i.e., 27% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 225,254 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 11th percentile – i.e., 11% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 3 others from the same source and published within six weeks on either side of this one.