| Title |
Characterization of a tryptophan 2-monooxygenase gene from Puccinia graminis f. sp. tritici involved in auxin biosynthesis and rust pathogenicity.
|
|---|---|
| Published in |
Molecular Plant-Microbe Interactions, March 2014
|
| DOI | 10.1094/mpmi-09-13-0289-fi |
| Pubmed ID | |
| Authors | |
| Abstract |
The plant hormone indole-3-acetic acid (IAA) is best known as a regulator of plant growth and development but its production can also affect plant-microbe interactions. Microorganisms, including numerous plant-associated bacteria and several fungi, are also capable of producing IAA. The stem rust fungus Puccinia graminis f. sp. tritici induced wheat plants to accumulate auxin in infected leaf tissue. A gene (Pgt-IaaM) encoding a putative tryptophan 2-monooxygenase, which makes the auxin precursor indole-3-acetamide (IAM), was identified in the P. graminis f. sp. tritici genome and found to be expressed in haustoria cells in infected plant tissue. Transient silencing of the gene in infected wheat plants indicated that it was required for full pathogenicity. Expression of Pgt-IaaM in Arabidopsis caused a typical auxin expression phenotype and promoted susceptibility to the bacterial pathogen Pseudomonas syringae pv. tomato DC3000. |
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
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| Germany | 2 | 29% |
| Chile | 1 | 14% |
| Unknown | 1 | 14% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 6 | 86% |
| Science communicators (journalists, bloggers, editors) | 1 | 14% |
Mendeley demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Australia | 2 | 2% |
| United States | 1 | 1% |
| Colombia | 1 | 1% |
| Unknown | 77 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 21 | 26% |
| Researcher | 13 | 16% |
| Student > Master | 10 | 12% |
| Professor | 6 | 7% |
| Student > Doctoral Student | 5 | 6% |
| Other | 14 | 17% |
| Unknown | 12 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 41 | 51% |
| Biochemistry, Genetics and Molecular Biology | 18 | 22% |
| Environmental Science | 3 | 4% |
| Business, Management and Accounting | 1 | 1% |
| Computer Science | 1 | 1% |
| Other | 2 | 2% |
| Unknown | 15 | 19% |