Xenopus

Chapter 1 Best Practices in Xenopus Housing, Husbandry, and the 3Rs

Chapter 2 Producing Xenopus Eggs and Embryos

Chapter 3 Xenbase: A Guide to the Xenopus Genetics and Genomics Knowledgebase

Chapter 4 mRNA Microinjection for Gene Expression Studies in Xenopus laevis and Xenopus tropicalis

Chapter 5 CRISPR-Mediated Gene Editing: Generating Gene Knockouts or Precision Base Changes in F 0 Xenopus

Chapter 6 Generation of Xenopus Transgenic Lines Using I-SceI

Chapter 7 Xenopus laevis Egg Extracts

Chapter 8 Cryogenic Preservation of Xenopus Sperm

Chapter 9 Adeno-Associated Virus (AAV) for Tracking Neural Development and Connectivity in Xenopus

Chapter 10 Whole Mount In Situ Hybridization in Xenopus

Chapter 11 Fluorescent In Situ mRNA Hybridization (FISH) Using Hybridization Chain Reaction (HCR) in Xenopus Cryosections

Chapter 12 Immunolocalization in Xenopus

Chapter 13 In Vivo Wide-Field Fluorescence Ca 2+ Imaging of Xenopus Tadpole Brains

Chapter 14 Blood–Brain Barrier Disruption and Edema in Xenopus: Methods for Analysis

Chapter 15 Automated Behavioral Tracking and Analysis of Xenopus Tadpoles

Chapter 16 Working Memory Y-Maze Test for Xenopus Tadpoles

Chapter 17 Producing Gynogenetic Embryos in Xenopus tropicalis

Chapter 18 Biomechanical Studies with Xenopus Animal Caps: A 3D-Printed Mechanical Bracket for Maintaining Tissue Stretch During Immunofluorescence Analysis

Chapter 19 Direct Observation and Measurement of Membrane Tension in Xenopus laevis Animal Cap Explants Using the Mechanosensitive Dye, Flipper-TR

Chapter 20 Spinal Cord Regeneration in Xenopus

Chapter 21 Retinal Degeneration and Regeneration in Xenopus laevis Tadpoles

Chapter 22 Determining Sex in Xenopus laevis Tadpoles

Chapter 23 Bone and Cartilage Staining for Skeletal Preparations