The peptide hormone calcitonin is intimately connected with human cancer development and proliferation. Its biosynthesis is reasoned to proceed via glycine-, α-hydroxyglycine-, glycyllysine- and glycyllysyllysine-extended precursors though, as a result of limitations of current analytical methods, until now there has been no procedure capable of detecting these individual species in cell or tissue samples. Therefore their presence and dynamics in cancer had not been established. Here we report the first methodology for the separation, detection and quantification of calcitonin and each of these precursors in human cancer cells. We also report the discovery and characterization of O-glycosylated calcitonin and its analogous biosynthetic precursors. Through direct and simultaneous analysis of the glycosylated and non-glycosylated species, we interrogate the hormone biosynthesis, to observe that the cellular calcitonin level is maintained to mitigate effects of biosynthetic enzyme inhibitors that substantially change the proportions of calcitonin-related species released into the culture medium.
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 June 2019.
All research outputs
#8,895,025
of 27,829,204 outputs
Outputs from Analytical Chemistry
#9,455
of 29,754 outputs
Outputs of similar age
#121,527
of 339,275 outputs
Outputs of similar age from Analytical Chemistry
#77
of 273 outputs
Altmetric has tracked 27,829,204 research outputs across all sources so far. This one has received more attention than most of these and is in the 67th percentile.
So far Altmetric has tracked 29,754 research outputs from this source. They receive a mean Attention Score of 5.0. This one has gotten more attention than average, scoring higher than 66% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 339,275 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 63% of its contemporaries.
We're also able to compare this research output to 273 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 67% of its contemporaries.
