When I did a genetic screen for machinery involved in recycling of surface proteins, I wanted to use that assay to validate hits. https://t.co/gcxQSoFU4Y It worked, but it was hard as I had to knockout each of the 89 candidates in a Tryptophan auxotroph…
11/ Helpfully, I had previously used the recycling reporter to screen all non-essential yeast deletions for recycling machinery candidates. https://t.co/nhph2ZwkiB Some of these had functional connections with glucose metabolism, (Gpa1, Gpr1, Ras2) in…
RT @RockUPress: Ste3-GFP-DUb was localized in yeast cells grown under identical conditions —MacDonald and Piper @JCellBiol https://t.co/Pcp…
RT @RockUPress: Ste3-GFP-DUb was localized in yeast cells grown under identical conditions —MacDonald and Piper @JCellBiol https://t.co/Pcp…