Herein, we report a method for in vitro selection of multivalent glycopeptides, combining mRNA display with incorporation of unnatural amino acids and "click" chemistry. We have demonstrated the use of this method to design potential glycopeptide vaccines against HIV. From libraries of ~10(13) glycopeptides containing multiple Man9 glycan(s), we selected variants that bind to HIV broadly neutralizing antibody 2G12 with picomolar to low nanomolar affinity. This is comparable to the strength of the natural 2G12-gp120 interaction, and is the strongest affinity achieved to date with constructs containing 3-5 glycans. These glycopeptides are therefore of great interest in HIV vaccine design.
Pharmacology, Toxicology and Pharmaceutical Science
6
6%
Engineering
3
3%
Other
10
10%
Unknown
21
21%
Attention Score in Context
Attention Score in Context
This research output has an Altmetric Attention Score of 16. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 August 2023.
All research outputs
#2,841,636
of 34,300,846 outputs
Outputs from Journal of the American Chemical Society
#7,097
of 90,101 outputs
Outputs of similar age
#24,037
of 267,543 outputs
Outputs of similar age from Journal of the American Chemical Society
#68
of 547 outputs
Altmetric has tracked 34,300,846 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 91st percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 90,101 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.7. This one has done particularly well, scoring higher than 91% of its peers.
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We're also able to compare this research output to 547 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 87% of its contemporaries.
