Nanozymes are an excellent class of optical reporters for the development of sensitive biosensors for widespread applications. In this study, platinum-palladium (Pt-Pd) nanoparticles were applied as signal amplifier in a dual-lateral flow immunoassays (LFIA) and integrated with a smartphone-based device for use in simultaneous detection of Salmonella Enteritidis and E. coli O157:H7. After optimization, the LODs were calculated to be ∼20 CFU/mL for Salmonella Enteritidis and ∼34 CFU/mL for E. coli O157:H7, respectively. The greatly improved sensitivity was contributed by the peroxidase-like catalytic activity of the Pt-Pd nanoparticles for signal enhancement and the parallel design of dual-detection for noninterference. The estimated recoveries of the dual-LFIA range from 91.44% to 117.00%, which indicated the developed method is capable of detecting live bacteria in food samples. This approach provides an attractive platform for Salmonella Enteritidis and E. coli O157:H7 detection using a smartphone-based device as the sole piece of equipment, indicating great promise for foodborne pathogen analysis or in-field food safety tracking.