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Mendeley readers
| Title |
Efficient stabilization of Saccharomyces cerevisiae external invertase by immobilisation on modified beidellite nanoclays
|
|---|---|
| Published in |
Food Chemistry, July 2014
|
| DOI | 10.1016/j.foodchem.2014.07.055 |
| Pubmed ID | |
| Authors |
Uroš Andjelković, Aleksandra Milutinović-Nikolić, Nataša Jović-Jovičić, Predrag Banković, Teja Bajt, Zorica Mojović, Zoran Vujčić, Dušan Jovanović |
| Abstract |
The external invertase isoform 1 (EINV1) was immobilised on eight differently modified beidellite nanoclays. Modifications were composed of organo-modification with different amounts of surfactant - hexadecyl trimethylammonium cation (HDTMA), pillaring with Al/Fe containing polyhydroxy cations and acid modification of Na-enriched and pillared clays. The modified nanoclays were characterised by XRD, N2-physisorption, SEM and FT-IR spectroscopy. The amount of bound enzyme activity was significantly influenced by the modification of beidellite ranging from 50 to remarkable 2200U/g. Biochemical characterization was performed for five modified nanoclays showing the highest enzyme activity after invertase immobilisation. The investigation demonstrated that after immobilisation the structure and the catalytic properties of invertase were preserved, while Km values were slightly increased from 26 to 37mM. immobilisation significantly improved thermal and storage stability of EINV1. Results indicate that beidellite nanoclays obtained by low cost modifications can be applied as a suitable support for the immobilisation of invertase. The immobilizate can be efficiently engaged in sucrose hydrolysis in batch reactor. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 55 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Serbia | 1 | 2% |
| Unknown | 54 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 9 | 16% |
| Researcher | 7 | 13% |
| Student > Ph. D. Student | 6 | 11% |
| Student > Master | 6 | 11% |
| Professor > Associate Professor | 4 | 7% |
| Other | 12 | 22% |
| Unknown | 11 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 11 | 20% |
| Biochemistry, Genetics and Molecular Biology | 9 | 16% |
| Chemistry | 7 | 13% |
| Engineering | 7 | 13% |
| Chemical Engineering | 2 | 4% |
| Other | 5 | 9% |
| Unknown | 14 | 25% |