Title |
Two Low Coverage Bird Genomes and a Comparison of Reference-Guided versus De Novo Genome Assemblies
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Published in |
PLOS ONE, September 2014
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DOI | 10.1371/journal.pone.0106649 |
Pubmed ID | |
Authors |
Daren C. Card, Drew R. Schield, Jacobo Reyes-Velasco, Matthew K. Fujita, Audra L. Andrew, Sara J. Oyler-McCance, Jennifer A. Fike, Diana F. Tomback, Robert P. Ruggiero, Todd A. Castoe |
Abstract |
As a greater number and diversity of high-quality vertebrate reference genomes become available, it is increasingly feasible to use these references to guide new draft assemblies for related species. Reference-guided assembly approaches may substantially increase the contiguity and completeness of a new genome using only low levels of genome coverage that might otherwise be insufficient for de novo genome assembly. We used low-coverage (∼3.5-5.5x) Illumina paired-end sequencing to assemble draft genomes of two bird species (the Gunnison Sage-Grouse, Centrocercus minimus, and the Clark's Nutcracker, Nucifraga columbiana). We used these data to estimate de novo genome assemblies and reference-guided assemblies, and compared the information content and completeness of these assemblies by comparing CEGMA gene set representation, repeat element content, simple sequence repeat content, and GC isochore structure among assemblies. Our results demonstrate that even lower-coverage genome sequencing projects are capable of producing informative and useful genomic resources, particularly through the use of reference-guided assemblies. |
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Geographical breakdown
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United States | 3 | 43% |
Canada | 1 | 14% |
Unknown | 3 | 43% |
Demographic breakdown
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Scientists | 6 | 86% |
Members of the public | 1 | 14% |
Mendeley readers
Geographical breakdown
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Germany | 2 | 2% |
Brazil | 2 | 2% |
Netherlands | 1 | <1% |
Vietnam | 1 | <1% |
Portugal | 1 | <1% |
France | 1 | <1% |
Australia | 1 | <1% |
Unknown | 99 | 89% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 26 | 23% |
Student > Bachelor | 13 | 12% |
Student > Master | 10 | 9% |
Student > Doctoral Student | 7 | 6% |
Other | 9 | 8% |
Unknown | 17 | 15% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 14 | 13% |
Environmental Science | 3 | 3% |
Nursing and Health Professions | 1 | <1% |
Computer Science | 1 | <1% |
Other | 1 | <1% |
Unknown | 21 | 19% |