Cellular senescence is emerging as a key mechanism of age-related vascular endothelial dysfunction, but evidence in healthy humans is lacking. Moreover, the influence of lifestyle factors such as habitual exercise on endothelial cell (EC) senescence is unknown. We tested the hypothesis that EC senescence increases with sedentary, but not physically active, aging and is associated with vascular endothelial dysfunction. Protein expression (quantitative immunofluorescence) of p53, a transcription factor related to increased cellular senescence, and the cyclin dependent kinase inhibitors p21 and p16 were 116%, 119%, and 128% greater (all P<0.05), respectively, in ECs obtained from antecubital veins of older sedentary (n=12, 60±1 yrs) vs. young sedentary (n=9, 22±1 yrs) adults. These age-related differences were not present (all P>0.05) in venous ECs from older exercising adults (n=13, 57±1 yrs). Furthermore, venous EC protein levels of p53 (r=-0.49, P=0.003), p21 (r=-0.38, P=0.03), and p16 (r=-0.58, P=0.002) were inversely associated with vascular endothelial function (brachial artery flow-mediated dilation). Similarly, p53 and p21 protein expression were 26% and 23% higher (both P<0.05), respectively, in ECs sampled from brachial arteries of healthy older sedentary (n=18, 63±1 yrs) vs. young sedentary (n=9, 25±1 yrs) adults; age-related changes in arterial EC p53 and p21 expression were not observed (P>0.05) in older habitually exercising adults (n=14, 59±1 yrs). These data indicate that EC senescence is associated with sedentary aging and is linked to endothelial dysfunction. Moreover, these data suggest that prevention of EC senescence may be one mechanism by which aerobic exercise protects against endothelial dysfunction with age.
Pharmacology, Toxicology and Pharmaceutical Science
6
3%
Other
22
12%
Unknown
74
42%
Attention Score in Context
Attention Score in Context
This research output has an Altmetric Attention Score of 21. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 April 2019.
All research outputs
#2,293,455
of 34,297,219 outputs
Outputs from American Journal of Physiology: Heart & Circulatory Physiology
#239
of 7,389 outputs
Outputs of similar age
#33,501
of 359,931 outputs
Outputs of similar age from American Journal of Physiology: Heart & Circulatory Physiology
#8
of 75 outputs
Altmetric has tracked 34,297,219 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 93rd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 7,389 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.2. This one has done particularly well, scoring higher than 96% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 359,931 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 90% of its contemporaries.
We're also able to compare this research output to 75 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 89% of its contemporaries.
