Title |
Adhesion of human periodontal ligament cells by three-dimensional culture to the sterilized root surface of extracted human teeth
|
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Published in |
Journal of Oral Science, January 2017
|
DOI | 10.2334/josnusd.16-0501 |
Pubmed ID | |
Authors |
Chieko Mino, Toshio Iwata, Toshitsugu Kawata |
Abstract |
Residual periodontal ligament (PDL) and cement mass on the roots of extracted teeth are factors that considerably affect tooth transplantation. Therefore, when normal extracted teeth are used for autologous transplantation, it is necessary to regenerate the PDL of the root surface. Here we describe a method to examine human PDL cell adhesion on sterilized root surfaces. Sample teeth were extracted during orthodontic treatment. PDL cells were obtained from healthy periodontal tissue explants from teeth extracted for orthodontic reasons. We developed a method for adhering PDL cells to sterile root surfaces using three-dimensional culture for 3 weeks. We evaluated the adhesion of human PDL cells to the sterilized root surfaces biochemically and histologically. The adherent PDL cells presented new projections on the sterile root surfaces. Therefore, PDL cells can adhere to sterile root surfaces. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Saudi Arabia | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Practitioners (doctors, other healthcare professionals) | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 21 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 2 | 10% |
Student > Ph. D. Student | 2 | 10% |
Student > Master | 2 | 10% |
Professor | 2 | 10% |
Other | 1 | 5% |
Other | 2 | 10% |
Unknown | 10 | 48% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 7 | 33% |
Biochemistry, Genetics and Molecular Biology | 1 | 5% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 5% |
Social Sciences | 1 | 5% |
Agricultural and Biological Sciences | 1 | 5% |
Other | 0 | 0% |
Unknown | 10 | 48% |