The essential oil, different extracts and isolated compounds of Angelica pancicii Vandas (Apiaceae) were investigated for the first time. The GC-FID and GC-MS analyses revealed sesquiterpenoids as the main constituents of A. pancicii essential oil of aerial parts with bornyl acetate (8.08%), n-octanol (5.82%), kessane (4.26%) and β-selinene (4.26%) as the main constituents. Analysis of methanol extracts, using HPLC-DAD/ESI-ToF-MS system, showed a total of 52 compounds in the aerial parts and 53 in the roots, indicated coumarins as the main constituents. In addition, new chromone (1) and six known furanocoumarins (2‒7) were isolated from the roots and structurally elucidated by combined spectroscopic methods. The aerial parts extracts exhibited higher polyphenolic contents and antioxidant activity evaluated by three radical scavenging assays. Using micro-well dilution method, the strongest antibacterial activity profiles were determined for ethanol and methanol root extracts (minimum bactericidal concentrations (MBCs) = 0.25‒3.00 mg/mL), which were comparable to the activity of streptomycin (MBCs = 0.34‒1.24 mg/mL), while the strongest antibacterial compound of A. pancicii was oxypeucedanin hydrate (MBCs = 0.50‒8.00 mg/mL). Antifungal potential was in moderate extent and the highest activity was obtained for roots methanol extract (minimum fungicidal concentrations (MFCs) = 4.00-14.00 mg/mL).Tested sub-minimum inhibitory concentrations (subMICs) of the extracts and isolated compounds inhibited selected Pseudomonas aeruginosa PAO1 virulence determinants. The most reduced growth of P. aeruginosa colony was in the presence of isolated oxypeucedanin. Ethanol (17.36‒46.98%) and methanol (34.54‒52.43%) roots extracts showed higher anti-biofilm activity compared to streptomycin (49.40‒88.36%) and ampicillin (56.46‒92.16%).