Context: Polycystic ovary syndrome (PCOS), a major cause of anovulatory infertility, is characterized by arrested follicular growth. Altered protein levels in the follicular fluid surrounding the ovum may reflect the molecular defects of folliculogenesis in these women. Objective: s: To identify differentially regulated proteins in PCOS by comparing follicular fluid protein repertoire of PCOS with healthy women. Methods: The follicular fluid samples were collected from PCOS and normo-ovulatory women undergoing in vitro fertilization. Follicular fluid proteins were subjected to digestion using trypsin and resultant peptides were labeled with iTRAQ reagents and analyzed by liquid chromatography tandem mass spectrometry. Differential abundance of selected proteins was confirmed by ELISA. Results: A total of 770 proteins were identified of which 186 showed differential abundance between controls and women with PCOS. Proteins involved in various processes of follicular development including amphiregulin; heparan sulfate proteoglycan 2; tumor necrosis factor, alpha-induced protein 6; plasminogen and lymphatic vessel endothelial hyaluronan receptor 1 were found to be deregulated in PCOS. We also identified a number of new proteins from follicular fluid, whose function in the ovary is not yet clearly established. These include suprabasin; S100 calcium binding protein A7 and helicase with zinc finger 2, transcriptional coactivator. Conclusions: Proteins indispensible for follicular growth were found to be differentially expressed in follicular fluid of women with PCOS, which may in part explain the aberrant folliculogenesis observed in these women.
This research output has an Altmetric Attention Score of 17. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 December 2023.
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#2,784,844
of 34,559,526 outputs
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#2,763
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#26,177
of 302,558 outputs
Outputs of similar age from JCEM
#30
of 176 outputs
Altmetric has tracked 34,559,526 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 91st percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 21,103 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.9. This one has done well, scoring higher than 86% of its peers.
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We're also able to compare this research output to 176 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 82% of its contemporaries.
