Background:Club cell secretory protein (CCSP) was found as a protective biomarker associated with annual decline in lung function. COPD progression results from an imbalance between injury and repair initially triggered by cigarette smoking. Objective:We investigated the effect of CCSP as a therapeutic strategy restoring the balance between injury and repair in COPD simultaneously validating an ex vivo air liquid interface (ALI) culture of human bronchial epithelial cells. Methods:Endobronchial biopsies (EBB) have been obtained from 13 COPD patients, 8 smokers and 8 control subjects. Morphometric analysis of the initial EBB has been performed. ALI cultures derived from the same EBB were exposed to cigarette smoke extract (CSE) with or without exogenous rhCCSP supplementation. CCSP and CXCL8 concentrations were assessed at steady state and after CSE exposure. Results:Morphometric analysis of the initial EBB showed increased cell density but decreased immunostaining of CCSP+ cells in COPD (p=0.03 vs. controls). At steady state, lower CCSP (p=0.04) and higher CXCL8 levels (p<.0001) were found in COPD ALI epithelium. Exogenous rhCCSP supplementation dampened CSE-induced CXCL8-release in COPD patients which returned to similar levels as smokers and controls (p=.0001). A negative correlation was found between CXCL8-release in ALI and CCSP+ cells density in initial biopsies (p=.0073). Conclusion:In vitro, rhCCSP exogenous supplementation can reverse CSE-induced CXCL8-release in COPD indicating a potential use of this strategy in vivo.
This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 March 2016.
All research outputs
#10,179,959
of 34,359,530 outputs
Outputs from CHEST
#8,961
of 18,088 outputs
Outputs of similar age
#92,068
of 307,953 outputs
Outputs of similar age from CHEST
#65
of 107 outputs
Altmetric has tracked 34,359,530 research outputs across all sources so far. This one has received more attention than most of these and is in the 70th percentile.
So far Altmetric has tracked 18,088 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 13.1. This one has gotten more attention than average, scoring higher than 50% of its peers.
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We're also able to compare this research output to 107 others from the same source and published within six weeks on either side of this one. This one is in the 38th percentile – i.e., 38% of its contemporaries scored the same or lower than it.
