| Chapter title |
CRISPR Repair Reveals Causative Mutation in a Preclinical Model of Retinitis Pigmentosa: A Brief Methodology
|
|---|---|
| Chapter number | 13 |
| Book title |
Retinal Gene Therapy
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7522-8_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7521-1, 978-1-4939-7522-8
|
| Authors |
Wen-Hsuan Wu, Yi-Ting Tsai, Sally Justus, Galaxy Y. Cho, Jesse D. Sengillo, Yu Xu, Thiago Cabral, Chyuan-Sheng Lin, Alexander G. Bassuk, Vinit B. Mahajan, Stephen H. Tsang, Wu, Wen-Hsuan, Tsai, Yi-Ting, Justus, Sally, Cho, Galaxy Y., Sengillo, Jesse D., Xu, Yu, Cabral, Thiago, Lin, Chyuan-Sheng, Bassuk, Alexander G., Mahajan, Vinit B., Tsang, Stephen H. |
| Abstract |
CRISPR/Cas9 genome engineering is currently the leading genome surgery technology in most genetics laboratories. Combined with other complementary techniques, it serves as a powerful tool for uncovering genotype-phenotype correlations. Here, we describe a simplified protocol that was used in our publication, CRISPR Repair Reveals Causative Mutation in a Preclinical Model of Retinitis Pigmentosa, providing an overview of each section of the experimental process. |
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X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 27 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 6 | 22% |
| Professor | 4 | 15% |
| Researcher | 2 | 7% |
| Other | 1 | 4% |
| Student > Doctoral Student | 1 | 4% |
| Other | 2 | 7% |
| Unknown | 11 | 41% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 15% |
| Agricultural and Biological Sciences | 4 | 15% |
| Medicine and Dentistry | 2 | 7% |
| Philosophy | 1 | 4% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
| Other | 3 | 11% |
| Unknown | 12 | 44% |