Rationale: NADPH oxidase is responsible for respiratory burst in immune cells. Chloride channel-3 (CLC3) has been linked to the respiratory burst in eosinophils and neutrophils. Effect of cytokines and involvement of CLC3 in the regulation of NADPH-dependent oxidative stress and on cytokine-mediated migration of eosinophils is not known. Methods: Human peripheral blood eosinophils were isolated from healthy and asthmatic individuals by negative selection. Real-time PCR was used to detect the expression of NADPH oxidases in eosinophils. Intracellular ROS measurement was done with flow cytometry. Superoxide generation was measured with TGF-β, eotaxin, and CLC3 blockers. CLC3 dependence of eosinophils in TGF-β- and eotaxin-induced migration was also examined. Results: The mRNA transcripts of NOX2, DUOX1 and DUOX2 were detected in blood eosinophils with very low expression of NOX1, NOX3 and NOX5, and no NOX4 mRNA. The level of NOX2 mRNA transcripts increased with disease severity in the eosinophils of asthmatics compared to healthy non-atopic volunteers. Change in granularity and size in eosinophils, but no change in intracellular ROS was observed with PMA. PMA, TGF-β and eotaxin utilized CLC3-dependent pathway to increase superoxide radicals. Both TGF-β and eotaxin induced CLC3-dependent chemotaxis of eosinophils. Conclusion: These findings support the requirement of CLC3 in the activation and migration of human blood eosinophils, and may provide potential novel therapeutic target to regulate eosinophil hyperactivity in allergic airway inflammation in asthma.
This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 November 2022.
All research outputs
#5,983,013
of 34,763,505 outputs
Outputs from American Journal of Respiratory Cell and Molecular Biology
#719
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Outputs of similar age
#55,709
of 301,624 outputs
Outputs of similar age from American Journal of Respiratory Cell and Molecular Biology
#4
of 43 outputs
Altmetric has tracked 34,763,505 research outputs across all sources so far. Compared to these this one has done well and is in the 82nd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,516 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.0. This one has done well, scoring higher than 84% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 301,624 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 81% of its contemporaries.
We're also able to compare this research output to 43 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 90% of its contemporaries.
