The generation of beta-thalassemia (β-Thal) patient-specific induced pluripotent stem cells (iPSCs), subsequent homologous recombination-based gene correction of disease-causing mutations/deletions in the β-globin gene (HBB) and their derived hematopoietic stem cells (HSCs) transplantation offers an ideal therapeutic solution for treating this disease. However, the hematopoietic differentiation efficiency of gene-corrected β-Thal iPSCs has not been well evaluated in the previous studies. In this study, we used the latest gene-editing tool CRISPR/Cas9 to correct β-Thal iPSCs, gene-corrected cells exhibit normal karyotypes and fully pluripotency as hES, showed no off-targeting effects. Then we evaluated the differentiation efficiency of the gene corrected β-Thal iPSCs. We found that during hematopoietic differentiation, gene-corrected β-Thal iPSCs showed increased embryoid body (EB) ratio and various hematopoietic progenitor cells percentages. More importantly, the gene-corrected β-Thal iPS cell lines restored HBB expression and reduced reactive oxygen species (ROS) production compared to un-corrected group. Our study suggested that hematopoietic differentiation efficiency of β-Thal iPSCs were greatly improved once corrected by CRISPR/Cas9 system. And the information gained from our study would greatly promote the clinical application of β-Thal iPSCs-derived HSCs in transplantation.
This research output has an Altmetric Attention Score of 18. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 October 2026.
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#2,650,571
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Outputs from Stem Cells & Development
#127
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#30,208
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Outputs of similar age from Stem Cells & Development
#3
of 44 outputs
Altmetric has tracked 34,364,397 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 92nd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,040 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 7.2. This one has done particularly well, scoring higher than 93% of its peers.
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We're also able to compare this research output to 44 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 93% of its contemporaries.
