Mycobacterial lipids play an important role in the modulation of the immune response upon contact with the host. Using novel methods, we have isolated highly purified PIM molecules (PIM2, AcPIM2, Ac2PIM2, AcPIM6 and Ac2PIM6) from virulent Mycobacterium tuberculosis to assess their potential to stimulate PBMC responses in M. bovis infected cattle. Of these molecules, 1 (AcPIM6) induced significant levels of IFN-γ in bovine PBMC. Three PIM molecules (AcPIM6, Ac2PIM2 and Ac2PIM6) were shown to drive significant proliferation in bovine PBMC. AcPIM6 was subsequently used to phenotype the proliferating cells by flow cytometry. This analysis demonstrated that AcPIM6 was predominantly recognised by CD3(+) CD335(+) NKT cells. In conclusion, we have identified PIM lipid molecules that interact with bovine lymphocyte populations and these lipids may be useful as future subunit vaccines or diagnostic reagents. Further, these data demonstrate, for the first time, lipid specific NKT activation in cattle.
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 February 2020.
All research outputs
#10,723,471
of 34,410,315 outputs
Outputs from Clinical and Vaccine Immunology (formerly CDLI)
#986
of 1,766 outputs
Outputs of similar age
#118,206
of 421,773 outputs
Outputs of similar age from Clinical and Vaccine Immunology (formerly CDLI)
#19
of 34 outputs
Altmetric has tracked 34,410,315 research outputs across all sources so far. This one has received more attention than most of these and is in the 67th percentile.
So far Altmetric has tracked 1,766 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 9.8. This one is in the 42nd percentile – i.e., 42% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 421,773 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.
We're also able to compare this research output to 34 others from the same source and published within six weeks on either side of this one. This one is in the 44th percentile – i.e., 44% of its contemporaries scored the same or lower than it.
