A knowledgeable workforce is key to meeting the demands of an aging population that is expected to nearly triple by 2030 and attitudes influence propensity to seek aging-related careers. Post-secondary education offers an opportunity to increase aging-related knowledge and facilitates intergenerational experiential-learning opportunities; however, research is lacking regarding attitudes towards aging and experiences among an academically diverse sample. To address this gap, the aims of this study were to: (1) examine differences in students' attitudes toward older adults by academic college; (2) assess differences in aging-related coursework and course experiences by academic college; and (3) explore key factors associated with students' attitudes toward older adults. Online survey methodology was used with a sample of 816 undergraduate students from a single university who represented all academic colleges offering undergraduate degrees. Significant differences among students' attitudes toward older adults by academic college were noted: those enrolled in the College of Human Sciences reported better stereotype, separation, and affective attitudes. Furthermore, factors associated with students' aging-related attitudes were sex, affiliation with academic college, perception of 'old', contact frequency, and desire to learn aging-related content. We suggest both interdisciplinary and disciplinary-specific educational recommendations to improve students' attitudes toward older adults.