A potential "new player" in arteries for mediating shear stress responses is the Epithelial Sodium Channel (ENaC). ENaC's contribution as shear sensor in intact arteries, and particularly different types of arteries (conduit and resistance) is unknown. We investigated the role of ENaC in both conduit (carotid) and resistance (3rd order mesenteric) arteries isolated from C57Bl/6J mice. Vessel characteristics were determined at baseline (60 mmHg, no-flow), in response to increased intraluminal pressure and shear stress using a pressure myograph. These protocols were performed in the absence and presence of the ENaC inhibitor amiloride (10 µM) and following inhibition of endothelial nitric oxide synthase (eNOS) by L-Name (100 µM). Under no-flow conditions, amiloride increased the internal and external diameter of carotid (13{plus minus}2%, p<0.05), but not mesenteric (0.5{plus minus}0.9%, p>0.05) arteries. In response to increased intraluminal pressure amiloride had no effect on the internal diameter of either type of artery. However, amiloride affected the stress/strain curves of mesenteric arteries. With increased shear stress ENaC-dependent effects were observed in both arteries. In carotid arteries, amiloride augmented flow-mediated dilation (9.2{plus minus}5.3%) compared with control (no amiloride, 6.2{plus minus}3.3%; p<0.05). In mesenteric arteries amiloride induced a flow-mediated constriction (-11.5{plus minus}6.6%) compared with control (-2.2{plus minus}4.5%; p<0.05). L-Name mimicked the effect of ENaC inhibition and prevented further amiloride effects in both types of arteries. These observations indicate that ENaC contributes to shear sensing in conduit and resistance arteries. The ENaC-mediated effects were associated with NO production although may involve different - artery dependent - downstream signalling pathways.
Pharmacology, Toxicology and Pharmaceutical Science
1
6%
Nursing and Health Professions
1
6%
Sports and Recreations
1
6%
Other
0
0%
Unknown
7
41%
Attention Score in Context
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 May 2018.
All research outputs
#22,326,550
of 34,376,207 outputs
Outputs from American Journal of Physiology: Heart & Circulatory Physiology
#5,634
of 7,399 outputs
Outputs of similar age
#296,974
of 493,461 outputs
Outputs of similar age from American Journal of Physiology: Heart & Circulatory Physiology
#35
of 56 outputs
Altmetric has tracked 34,376,207 research outputs across all sources so far. This one is in the 33rd percentile – i.e., 33% of other outputs scored the same or lower than it.
So far Altmetric has tracked 7,399 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.2. This one is in the 22nd percentile – i.e., 22% of its peers scored the same or lower than it.
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