Normal phase liquid chromatography–electrospray ionization tandem mass spectrometry analysis of phospholipid molecular species in blood mononuclear cells: application to cystic fibrosis
Published in
Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences, October 2004
The use of HPLC coupled on-line with a mass spectrometer is a very powerful tool in order to analyze intact PLs molecular species (PMS) without the need of derivatization, thus decreasing the risk of artifacts formation. A normal-phase HPLC-ESI-MS-MS method has been developed in order to study the human blood mononuclear cell PMS composition. This method was applied to characterize PMS from seven CF subjects and from seven age-matched healthy subjects. More than 140 phospholipid molecular species from phosphatidylethanolamine (PE), plasmalogen phosphatidylethanolamine (pPE), phosphatidylinositol (PI), phosphatidylserine (PS), phosphatidylcholine (PC) and sphingomyelin (Sph) were identified and compared. Differences between the two groups were found in pPE (p16:0/22:6), pPE (p18:0/22:6), PE (16:0/20:4) and PC (16:0/18:2) which were significantly lower in CF subjects and in PC (16:0/16:1) which was significantly higher in CF subjects.
Pharmacology, Toxicology and Pharmaceutical Science
3
7%
Environmental Science
1
2%
Other
4
9%
Unknown
9
20%
Attention Score in Context
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 May 2011.
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#12,343,970
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Outputs from Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences
#1,681
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Outputs of similar age
#42,974
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Outputs of similar age from Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences
#20
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