| Title |
Stereoselective Bimolecular Phenoxy Radical Coupling by an Auxiliary (Dirigent) Protein Without an Active Center
|
|---|---|
| Published in |
Science, January 1997
|
| DOI | 10.1126/science.275.5298.362 |
| Pubmed ID | |
| Authors |
Laurence B. Davin, Huai-Bin Wang, Anastasia L. Crowell, Diana L. Bedgar, Diane M. Martin, Simo Sarkanen, Norman G. Lewis |
| Abstract |
The regio- and stereospecificity of bimolecular phenoxy radical coupling reactions, of especial importance in lignin and lignan biosynthesis, are clearly controlled in some manner in vivo; yet in vitro coupling by oxidases, such as laccases, only produce racemic products. In other words, laccases, peroxidases, and comparable oxidases are unable to control regio- or stereospecificity by themselves and thus some other agent must exist. A 78-kilodalton protein has been isolated that, in the presence of an oxidase or one electron oxidant, effects stereoselective bimolecular phenoxy radical coupling in vitro. Itself lacking a catalytically active (oxidative) center, its mechanism of action is presumed to involve capture of E-coniferyl alcohol-derived free-radical intermediates, with consequent stereoselective coupling to give (+)-pinoresinol. |
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X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Hong Kong | 1 | 20% |
| United Kingdom | 1 | 20% |
| Canada | 1 | 20% |
| Germany | 1 | 20% |
| Unknown | 1 | 20% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 4 | 80% |
| Members of the public | 1 | 20% |
Mendeley demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | <1% |
| France | 1 | <1% |
| Germany | 1 | <1% |
| Switzerland | 1 | <1% |
| Brazil | 1 | <1% |
| Unknown | 232 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 59 | 25% |
| Researcher | 45 | 19% |
| Student > Master | 26 | 11% |
| Student > Bachelor | 16 | 7% |
| Student > Doctoral Student | 14 | 6% |
| Other | 26 | 11% |
| Unknown | 52 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 69 | 29% |
| Chemistry | 48 | 20% |
| Biochemistry, Genetics and Molecular Biology | 35 | 15% |
| Chemical Engineering | 4 | 2% |
| Materials Science | 4 | 2% |
| Other | 15 | 6% |
| Unknown | 63 | 26% |