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Development and characterization of two cell lines from gills of Atlantic salmon

Overview of attention for article published in PLOS ONE, February 2018
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Title
Development and characterization of two cell lines from gills of Atlantic salmon
Published in
PLOS ONE, February 2018
DOI 10.1371/journal.pone.0191792
Pubmed ID
Authors

Mona C. Gjessing, Maria Aamelfot, William N. Batts, Sylvie L. Benestad, Ole B. Dale, Even Thoen, Simon C. Weli, James R. Winton

Abstract

Gill disease in Atlantic salmon, Salmo salar L., causes big losses in the salmon farming industry. Until now, tools to cultivate microorganisms causing gill disease and models to study the gill responses have been lacking. Here we describe the establishment and characterization of two cell lines from the gills of Atlantic salmon. Atlantic salmon gill cell ASG-10 consisted of cells staining for cytokeratin and e-cadherin and with desmosomes as seen by transmission electron microscopy suggesting the cells to be of epithelial origin. These structures were not seen in ASG-13. The cell lines have been maintained for almost 30 passages and both cell lines are fully susceptible to infection by infectious hematopoietic necrosis virus (IHNV), viral hemorrhagic septicemia virus (VHSV), infectious pancreatic necrosis virus (IPNV), Atlantic salmon reovirus TS (TSRV) and Pacific salmon paramyxovirus (PSPV). While infectious salmon anemia virus (ISAV) did not cause visible CPE, immunofluorescent staining revealed a sub-fraction of cells in both the ASG-10 and ASG-13 lines may be permissive to infection. ASG-10 is able to proliferate and migrate to close scratches in the monolayer within seven days in vitro contrary to ASG-13, which does not appear to do have the same proliferative and migratory ability. These cell lines will be useful in studies of gill diseases in Atlantic salmon and may represent an important contribution for alternatives to experimental animals and studies of epithelial-mesenchymal cell biology.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 48 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 48 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 10 21%
Student > Ph. D. Student 5 10%
Student > Bachelor 3 6%
Student > Postgraduate 3 6%
Student > Master 3 6%
Other 6 13%
Unknown 18 38%
Readers by discipline Count As %
Agricultural and Biological Sciences 9 19%
Veterinary Science and Veterinary Medicine 6 13%
Immunology and Microbiology 5 10%
Biochemistry, Genetics and Molecular Biology 4 8%
Medicine and Dentistry 2 4%
Other 5 10%
Unknown 17 35%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 February 2018.
All research outputs
#18,587,406
of 23,023,224 outputs
Outputs from PLOS ONE
#156,409
of 196,234 outputs
Outputs of similar age
#335,140
of 446,257 outputs
Outputs of similar age from PLOS ONE
#2,843
of 3,618 outputs
Altmetric has tracked 23,023,224 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 196,234 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.2. This one is in the 10th percentile – i.e., 10% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 446,257 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 14th percentile – i.e., 14% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 3,618 others from the same source and published within six weeks on either side of this one. This one is in the 7th percentile – i.e., 7% of its contemporaries scored the same or lower than it.