Using Arabidopsis seedlings, we identified a range of small fluorescent probes that entered the translocation stream and were unloaded at the root tip. These probes had absorbance/emission maxima ranging from 367/454 nm to 546/576 nm and represent a versatile toolbox for studying phloem transport. Of the probes we tested, naturally occurring fluorescent coumarin glucosides (esculin, fraxin) were phloem loaded, and were transported in oocytes by the sucrose transporter, AtSUC2. Arabidopsis plants in which AtSUC2 was replaced with barley sucrose transporter HvSUT1, which does not transport esculin, failed to load esculin into the phloem. In wild-type plants the fluorescence of esculin decayed to background levels after about 2h following phloem unloading, making it a suitable tracer for pulse-labelling studies of phloem transport. We identified additional probes, such as carboxytetraethylrhodamine; CTER) a red fluorescent probe that, unlike esculin, was stable for several hours following phloem unloading and could be used to study phloem transport in Arabidopsis lines expressing GFP.
This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 January 2020.
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#5,640,567
of 30,720,210 outputs
Outputs from Plant Physiology
#3,769
of 14,083 outputs
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#65,154
of 383,801 outputs
Outputs of similar age from Plant Physiology
#18
of 94 outputs
Altmetric has tracked 30,720,210 research outputs across all sources so far. Compared to these this one has done well and is in the 81st percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 14,083 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 7.1. This one has gotten more attention than average, scoring higher than 73% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 383,801 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 83% of its contemporaries.
We're also able to compare this research output to 94 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 80% of its contemporaries.
