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X Demographics
Mendeley readers
Attention Score in Context
| Title |
A real-time, quantitative PCR protocol for assessing the relative parasitemia of Leucocytozoon in waterfowl
|
|---|---|
| Published in |
Journal of Microbiological Methods, February 2015
|
| DOI | 10.1016/j.mimet.2015.01.027 |
| Pubmed ID | |
| Authors |
Matthew M. Smith, Joel Schmutz, Chloe Apelgren, Andrew M. Ramey |
| Abstract |
Microscopic examination of blood smears can be effective at diagnosing and quantifying hematozoa infections. However, this method requires highly trained observers, is time consuming, and may be inaccurate for detection of infections at low levels of parasitemia. To develop a molecular methodology for identifying and quantifying Leucocytozoon parasite infection in wild waterfowl (Anseriformes), we designed a real-time, quantitative PCR protocol to amplify Leucocytozoon mitochondrial DNA using TaqMan fluorogenic probes and validated our methodology using blood samples collected from waterfowl in interior Alaska during late summer and autumn (n=105). By comparing our qPCR results to those derived from a widely used nested PCR protocol, we determined that our assay showed high levels of sensitivity (91%) and specificity (100%) in detecting Leucocytozoon DNA from host blood samples. Additionally, results of a linear regression revealed significant correlation between the raw measure of parasitemia produced by our qPCR assay (Ct values) and numbers of parasites observed on blood smears (R(2)=0.694, P=0.003), indicating that our assay can reliably determine the relative parasitemia levels among samples. This methodology provides a powerful new tool for studies assessing effects of haemosporidian infection in wild avian species. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 40 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 5% |
| Lithuania | 1 | 3% |
| Unknown | 37 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 11 | 28% |
| Student > Ph. D. Student | 9 | 23% |
| Student > Master | 7 | 18% |
| Student > Bachelor | 2 | 5% |
| Student > Postgraduate | 2 | 5% |
| Other | 3 | 8% |
| Unknown | 6 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 19 | 48% |
| Environmental Science | 3 | 8% |
| Biochemistry, Genetics and Molecular Biology | 3 | 8% |
| Veterinary Science and Veterinary Medicine | 2 | 5% |
| Medicine and Dentistry | 2 | 5% |
| Other | 3 | 8% |
| Unknown | 8 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 December 2015.
All research outputs
#16,721,717
of 25,374,647 outputs
Outputs from Journal of Microbiological Methods
#1,609
of 2,350 outputs
Outputs of similar age
#211,295
of 360,372 outputs
Outputs of similar age from Journal of Microbiological Methods
#19
of 44 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,350 research outputs from this source. They receive a mean Attention Score of 3.7. This one is in the 28th percentile – i.e., 28% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 360,372 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 38th percentile – i.e., 38% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 44 others from the same source and published within six weeks on either side of this one. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.