Linear-After-The-Exponential (LATE)-PCR: Primer design criteria for high yields of specific single-stranded DNA and improved real-time detection

Overview of attention for article published in Proceedings of the National Academy of Sciences of the United States of America, June 2005

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Today I learnt that the concentration of PCR primers used changes the Tm dramatically and this should be accounted for in asymmetric PCR. I’d always just ignored that field in the software #stilllearning #pcr #betterlatethannever https://t.co/ozKkzTfDuF

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