Epithelioid Haemangioendothelioma (EHE) is a malignant vascular neoplasm. subsets have been previously characterized by translocations resulting in either WWTR1-CAMTA1 or YAP1-TFE3 fusion. We sought to develop molecular and immunohistochemical (IHC) assays to aid in the diagnosis and characterization of EHE.
52 FFPE cases diagnosed between 2002 and 2014 were retrieved from the pathology files of our institutions. RT-PCR assays were optimized to detect WWTR1-CAMTA1 and YAP1-TFE3 fusion transcripts in FFPE tissue and TFE3 protein accumulation was examined by IHC. RNA was extracted from 33 adequate samples, with more recent cases providing a greater yield of high quality RNA. 14/18 informative cases were positive for WWTR1-CAMTA1 fusion transcripts, four of which showed higher grade cytological features termed by some as "malignant EHE". Novel in-frame fusion transcripts were identified in four cases by direct sequencing. IHC revealed variable nuclear TFE3 staining in 6/17 cases; three with patchy staining showed WWTR1-CAMTA1 fusion. 1 of 18 informative cases was positive for YAP1-TFE3 fusion and showed strong nuclear TFE3 staining by IHC.
This study confirms the high incidence of WWTR1-CAMTA1 and YAP1-TFE3 rearrangements in EHE and indicates that the staining pattern for TFE3 IHC is critical for specificity. This article is protected by copyright. All rights reserved.
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 July 2024.
All research outputs
#6,078,912
of 29,400,575 outputs
Outputs from Histopathology
#788
of 4,002 outputs
Outputs of similar age
#63,974
of 285,094 outputs
Outputs of similar age from Histopathology
#6
of 57 outputs
Altmetric has tracked 29,400,575 research outputs across all sources so far. Compared to these this one has done well and is in the 79th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,002 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.1. This one has done well, scoring higher than 80% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 285,094 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 77% of its contemporaries.
We're also able to compare this research output to 57 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 89% of its contemporaries.
