Title |
Multi-point Scanning Two-photon Excitation Microscopy by Utilizing a High-peak-power 1042-nm Laser
|
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Published in |
Analytical Sciences, April 2015
|
DOI | 10.2116/analsci.31.307 |
Pubmed ID | |
Authors |
Kohei Otomo, Terumasa Hibi, Takashi Murata, Hirotaka Watanabe, Ryosuke Kawakami, Hiroshi Nakayama, Mitsuyasu Hasebe, Tomomi Nemoto |
Abstract |
The temporal resolution of a two-photon excitation laser scanning microscopy (TPLSM) system is limited by the excitation laser beam's scanning speed. To improve the temporal resolution, the TPLSM system is equipped with a spinning-disk confocal scanning unit. However, the insufficient energy of a conventional Ti:sapphire laser source restricts the field of view (FOV) for TPLSM images to a narrow region. Therefore, we introduced a high-peak-power Yb-based laser in order to enlarge the FOV. This system provided three-dimensional imaging of a sufficiently deep and wide region of fixed mouse brain slices, clear four-dimensional imaging of actin dynamics in live mammalian cells and microtubule dynamics during mitosis and cytokinesis in live plant cells. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 50% |
Members of the public | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 43 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 8 | 19% |
Student > Ph. D. Student | 5 | 12% |
Other | 4 | 9% |
Professor > Associate Professor | 4 | 9% |
Student > Master | 4 | 9% |
Other | 7 | 16% |
Unknown | 11 | 26% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 7 | 16% |
Neuroscience | 7 | 16% |
Physics and Astronomy | 7 | 16% |
Biochemistry, Genetics and Molecular Biology | 5 | 12% |
Engineering | 3 | 7% |
Other | 3 | 7% |
Unknown | 11 | 26% |