As C4-dicarboxylic acids could replace C4-petrochemicals, the reductive TCA pathway was overexpressed in Pichia pastoris for producing the C4-dicarboxylic acids. Three expression cassettes which carried pyruvate carboxylase gene (pc), cytoplasm malate dehydrogenase gene (mdh1) and retargeted fumarase gene (Tfum) were integrated into the chromosome DNA of Pichia pastoris GS115 respectively or jointly. Multicopy integrations were screened using qPCR for C4-dicarboxylic acid over-accumulation. The results showed that the highest titer in 96 hours of fumaric, malic and succinic acid (0.76, 42.28 and 9.42 g·L(-1)) was obtained by co-expression of pc and mdh1 in Pichia pastoris. This is the first report about multiple genes engineered Pichia pastoris for C4-dicarboxylic acids production. The strain Pp-PC-MDH1 has moreover a significant potential to produce malic acid through aerobic condition.
This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 June 2023.
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#4
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Altmetric has tracked 25,374,917 research outputs across all sources so far. This one has received more attention than most of these and is in the 71st percentile.
So far Altmetric has tracked 5,772 research outputs from this source. They receive a mean Attention Score of 4.5. This one has gotten more attention than average, scoring higher than 73% of its peers.
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